Overview

The Western blot test is a laboratory technique used to detect specific proteins in a sample. It involves separating proteins by size using gel electrophoresis, transferring them onto a membrane, and then using antibodies to detect the target protein. The test is commonly used in medical research and diagnosis, such as for detecting infections like HIV or Lyme disease. The Western blot test is a highly sensitive and specific technique for protein detection.

Who should do this test?

Individuals who are suspected to be infected with HIV: Western blot test is commonly used to confirm HIV infection in individuals who have tested positive on an initial screening test. It detects antibodies produced by the body in response to the virus.

Individuals who are suspected to have Lyme disease: Western blot test is also used to diagnose Lyme disease, which is caused by the bacteria Borrelia burgdorferi. The test detects antibodies produced by the body in response to the bacteria.

Individuals who are suspected to have hepatitis C: Western blot test is used to confirm the presence of hepatitis C virus antibodies in individuals who have tested positive on an initial screening test. It is used to differentiate between true positives and false positives.

What to do if the test is abnormal?

Consult with a medical professional: A person who has received abnormal results from a Western blot test should consult with a medical professional. The medical professional will be able to provide guidance on what the abnormal results mean and what steps need to be taken next.

Further testing: Depending on the results of the Western blot test, further testing may be necessary. Additional tests will help to determine the underlying cause of the abnormal results and will help to guide treatment.

Treatment: Once the underlying cause of the abnormal results is determined, treatment can begin. Treatment will be tailored to the specific condition that is causing the abnormal results and may include medications, lifestyle changes, or other interventions.

What is the normal range of Western blot test (HIV-1 and HIV-2)?

The normal range for adult males is *,the normal range for adult females is *