This test measures your Vitamin D levels by checking 25-Hydroxy Cholecalciferol (25-OH Vitamin D) in a single blood sample. Book the Vitamin D test at our lab in Pune or get free home sample pickup at locations near you.
The test is also called the 25-Hydroxy Vitamin D Test or 25-OH Vitamin D Test.
Check the sample report PDF for more details. The cost of the Vitamin D test in Pune is Rs.1900.
A Vitamin D test is a blood test that measures the level of 25-Hydroxy Vitamin D (25-OH Cholecalciferol) in your body. It reflects your overall vitamin D status and how well your bones and immune system are supported.
It is commonly advised for people with bone pain, muscle weakness, fatigue or frequent fractures, and to monitor those at risk of vitamin D deficiency.

The vitamin D test is recommended for the following groups of people:
Your doctor may advise a Vitamin D test when you have symptoms that suggest low vitamin D levels. Common signs include:
The vitamin D test is a simple blood test and needs no special preparation. It is quick and nearly painless.
To prepare for the test:
To give the sample:
The Vitamin D test report includes the following parameter:
| Sample Type | Blood |
| Time to Get Report | 12.00 Noon 1 day |
| Test Method | CLIA / CMIA |
| Normal Range | 25-OH Vitamin D : 20.0 - 50.0 ng/ml |
| Purpose of test | Checks vitamin D levels and bone health status |
| Intended for | Adults with suspected vitamin D deficiency or bone-related concerns |
| Fasting Required | No |
| Total Tests | 1 |
| Doctor's Prescription Required | No |
| Reports Available By | PDF available through WhatsApp, Email and Hardcopy (on request) |
| Other Names | 25 Hydroxy Cholecalciferol, 25-OH Vitamin D, Vitamin D3 Test |
The Vitamin D test measures the level of vitamin D in your blood. The table below lists the parameter along with its normal range.
| Group | Normal Range |
|---|---|
| Female | 20 – 50 |
| Male | 20 – 50 |
| Result | Meaning | Common Causes |
|---|---|---|
| Low Vitamin D (below 20 ng/ml) | Vitamin D deficiency (not enough vitamin D in the blood, which weakens bones and muscles) | Low sun exposure (too little sunlight on the skin, which the body needs to make vitamin D); poor dietary intake (eating too few vitamin D-rich foods like fish, eggs, and fortified milk); malabsorption (the gut not absorbing nutrients properly, as in coeliac disease or Crohn's disease) |
| Borderline Vitamin D (20 to 30 ng/ml) | Insufficiency (levels lower than ideal but not fully deficient, which may still affect bone health over time) | Limited outdoor time (spending most of the day indoors); darker skin tone (more melanin, which reduces vitamin D made from sunlight); older age (skin makes less vitamin D as people grow older) |
| High Vitamin D (above 50 ng/ml) | Vitamin D excess (too much vitamin D in the blood, which can raise calcium to harmful levels) | Over-supplementation (taking high-dose vitamin D pills for too long); excess fortified foods (consuming large amounts of vitamin D-enriched products); certain conditions like sarcoidosis (an inflammatory disease that makes the body produce extra vitamin D) |
The Vitamin D test measures 25-Hydroxy Vitamin D (25-OH Cholecalciferol) in your blood. It shows your body's vitamin D level and helps detect deficiency.
At Pathofast Lab in Pune, the Vitamin D test costs Rs.1900. This is our standard price for the 25-Hydroxy Vitamin D blood test.
In cities like Mumbai, Delhi, Bengaluru, Hyderabad and Chennai, prices are roughly Rs.900 to Rs.2500. These are approximate ranges and vary by lab.
The normal range is 20.0 to 50.0 ng/ml. Levels below 20 ng/ml usually indicate deficiency, while very high levels may suggest excess vitamin D.
No, fasting is not required for the Vitamin D test. You can give your blood sample at any time of day without special preparation.
A small blood sample is collected from a vein in your arm. The test is simple, quick and needs no special preparation.
Common symptoms include tiredness, bone and back pain, muscle weakness, frequent illness and low mood. Many people, however, have no obvious symptoms at all.
People with bone pain, fatigue, muscle weakness, limited sun exposure, or those over 50 may benefit. Your doctor can advise if you need testing.
At Pathofast Lab, Pune, your Vitamin D report is usually ready by 12.00 noon the next day after sample collection.
Despite plenty of sunshine, deficiency is common due to indoor lifestyles, sunscreen use, darker skin, air pollution and clothing that limits sun exposure.
Get regular sunlight, eat fortified foods, fatty fish and eggs, and take supplements if advised by your doctor. Retest as your doctor recommends.
Yes, the 25-Hydroxy Vitamin D blood test is the standard, reliable way to check vitamin D status. Results are accurate when done at a quality lab.
Pathofast Lab in Pune, is the best lab in Pune for accurate and reliable blood tests. Our lab is located conveniently in the center of Pune and we also offer home sample collection for the Vitamin D Test in various areas of Pune.
You can be assured of quality service and timely and accurate reports